Monitoring optoporated process on mammalian cells by real-time measurement of membrane resealing time.

ABSTRACT:

Significance

Resealing time based loading efficiency of optoporation is the key parameter for drug or gene delivery. This work describes a comparatively simple optical approach to directly measure the cell membrane resealing time of the gold nanoparticle mediated photoporation.

Aim

To establish a membrane potential detection optical system, which can provide a direct measurement of resealing time of the optoporated cells.

Approach

Voltage sensitive dye has been used to label the gold nanoparticle covered cell before laser activation and the resealing time was estimated from the voltage change due to the fluorescence light intensity change before and after laser activation. The approach has been validated by the simulated data based on diffusion model and Monte Carlo s

SUBMITTER: Du X 

PROVIDER: S-EPMC10308995 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature