Dataset Information
Interaction Between NOS3 and HMOX1 on Antihypertensive Drug Responsiveness in Preeclampsia.
Ontology highlight
ABSTRACT: Objective
We examined the interaction of polymorphisms in the genes heme oxygenase-1 (HMOX1) and nitric oxide synthase (NOS3) in patients with preeclampsia (PE) as well as the responsiveness to methyldopa and to total antihypertensive therapy.Methods
The genes HMOX1 (rs2071746, A/T) and NOS3 (rs1799983, G/T) were genotyped using TaqMan allele discrimination assays (Applied Biosystems, Foster City, CA, USA ), and the levels of enzyme heme oxygenase-1 (HO-1) were measured using enzyme-linked immunosorbent assay (ELISA).Results
We found interactions between genotypes of the HMOX-1 and NOS3 genes and responsiveness to methyldopa and that PE genotyped as AT presents lower levels of protein HO-1 compared with AA.Conclusion
We found interactions between the HMOX-1 and NOS3 genes and responsiveness to methyldopa and that the HMOX1 polymorphism affects the levels of enzyme HO-1 in responsiveness to methyldopa and to total antihypertensive therapy. These data suggest impact of the combination of these two polymorphisms on antihypertensive responsiveness in PE.
SUBMITTER: Sandrim VC
PROVIDER: S-EPMC10309231 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Publications
Interaction Between NOS3 and HMOX1 on Antihypertensive Drug Responsiveness in Preeclampsia.
Revista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia 20200619 8
<h4>Objective</h4> We examined the interaction of polymorphisms in the genes heme oxygenase-1 (<i>HMOX1</i>) and nitric oxide synthase (<i>NOS3</i>) in patients with preeclampsia (PE) as well as the responsiveness to methyldopa and to total antihypertensive therapy.<h4>Methods</h4> The genes <i>HMOX1</i> (rs2071746, A/T) and <i>NOS3</i> (rs1799983, G/T) were genotyped using TaqMan allele discrimination assays (Applied Biosystems, Foster City, CA, USA ), and the levels of enzyme heme oxygenase-1 ...[more]
