MetaNovo: An open-source pipeline for probabilistic peptide discovery in complex metaproteomic datasets.

ABSTRACT:

Background

Microbiome research is providing important new insights into the metabolic interactions of complex microbial ecosystems involved in fields as diverse as the pathogenesis of human diseases, agriculture and climate change. Poor correlations typically observed between RNA and protein expression datasets make it hard to accurately infer microbial protein synthesis from metagenomic data. Additionally, mass spectrometry-based metaproteomic analyses typically rely on focused search sequence databases based on prior knowledge for protein identification that may not represent all the proteins present in a set of samples. Metagenomic 16S rRNA sequencing only targets the bacterial component, while whole genome sequencing is at best an indirect measure of expressed proteomes. Here w

SUBMITTER: Potgieter MG 

PROVIDER: S-EPMC10310047 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature