MicroRNA-145 and microRNA-486 are potential serum biomarkers for vascular calcification.

ABSTRACT:

Introduction

MicroRNAs (miRs) regulate vascular calcification (VC), and their quantification may contribute to suspicion of the presence of VC.

Methods

The study was performed in four phases. Phase 1: miRs sequencing of rat calcified and non-calcified aortas. Phase 2: miRs with the highest rate of change, plus miR-145 [the most abundant miR in vascular smooth muscle cells (VSMCs)], were validated in aortas and serum from rats with and without VC. Phase 3: the selected miRs were analyzed in epigastric arteries from kidney donors and recipients, and serum samples from general population. Phase 4: VSMCs were exposed to different phosphorus concentrations, and miR-145 and miR-486 were overexpressed to investigate their role in VC.

Results

miR-145, miR-122-5p, miR-486 and

SUBMITTER: Fernandez-Villabrille S 

PROVIDER: S-EPMC10310514 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature