Investigation of osmotic shock effect on pulsed electric field treated S. cerevisiae yeast cells.

ABSTRACT: Pulsed electric field (PEF) treatment is known to cause plasma membrane permeabilization of microorganisms, an effect known as electroporation. PEF treatment is very attractive since it can achieve permeabilization with or without lethal damage in accordance with desired results. This study aimed to expand the accomplishment of electroporation outcomes by applying sudden post-PEF osmotic composition change of the media. Changes in yeast cells' viability, size and plasma membrane regeneration rate were evaluated. However, we still have questions about the intracellular biochemical processes responsible for plasma membrane recovery after electroporation. Our suggested candidate is the high osmolarity glycerol (HOG) kinase pathway. The HOG pathway in Saccharomyces cerevisiae yeasts is respons

SUBMITTER: Gancyte G 

PROVIDER: S-EPMC10310692 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature