Single cell Hi-C identifies plastic chromosome conformations underlying the gastrulation enhancer landscape.

ABSTRACT: Embryonic development involves massive proliferation and differentiation of cell lineages. This must be supported by chromosome replication and epigenetic reprogramming, but how proliferation and cell fate acquisition are balanced in this process is not well understood. Here we use single cell Hi-C to map chromosomal conformations in post-gastrulation mouse embryo cells and study their distributions and correlations with matching embryonic transcriptional atlases. We find that embryonic chromosomes show a remarkably strong cell cycle signature. Despite that, replication timing, chromosome compartment structure, topological associated domains (TADs) and promoter-enhancer contacts are shown to be variable between distinct epigenetic states. About 10% of the nuclei are identified as primitive

SUBMITTER: Rappoport N 

PROVIDER: S-EPMC10310791 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature