Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis.

ABSTRACT:

Objective

To determine the effects of sperm deoxyribonucleic acid (DNA) fragmentation at the time of fertilization on in vitro fertilization (IVF) outcomes and genetic diagnosis using next generation sequencing.

Design

Prospective double-blinded study.

Setting

Private Clinic.

Patients

Couples (n = 150).

Intervention

In vitro fertilization with preimplantation genetic testing for aneuploidy and sperm DNA fragmentation assay, as in sperm chromatin structure assay the day of retrieval.

Main outcome measures

Laboratory outcomes are listed in the results section. Statistical analysis was performed using JMP, XYLSTAT, and STATA version 15.

Results

The sperm DNA fragmentation index (DFI) in the neat ejaculate did not predict fertilization rate, qu

SUBMITTER: Broussard AL 

PROVIDER: S-EPMC10310935 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature