Emergent structural and functional properties of hippocampal multi-cellular aggregates.

ABSTRACT: Hippocampal neural networks are distinctly capable of integrating multi-modal sensory inputs to drive memory formation. Neuroscientific investigations using simplified in vitro models have greatly relied on planar (2D) neuronal cultures made from dissociated tissue. While these models have served as simple, cost-effective, and high-throughput tools for examining various morphological and electrophysiological characteristics of hippocampal networks, 2D cultures fail to reconstitute critical elements of the brain microenvironment that may be necessary for the emergence of sophisticated integrative network properties. To address this, we utilized a forced aggregation technique to generate high-density (>100,000 cells/mm³) multi-cellular three-dimensional aggregates using rod

SUBMITTER: Acero VP 

PROVIDER: S-EPMC10311220 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature