Optimization, fractional characterization, and antioxidant potential of exopolysaccharides from Levilactobacillus brevis NCCP 963 isolated from "kanji".

ABSTRACT: A novel exopolysaccharide (EPS) was obtained from Levilactobacillus brevis NCCP 963 isolated from a black carrot drink named "kanji". The culture conditions for maximum EPS yield were explored by the Plackett-Burman (PB) design and response surface methodology (RSM) along with the fractional characterization and antioxidant potential of EPSs. The PB design screened out five significant factors, namely, glucose, sucrose, tryptone, CaCl₂, and di-potassium phosphate out of eleven independent factors. The RSM indicated glucose and CaCl₂ as significant factors in EPS production and a maximum EPS production of 968.89 mg L^-1 was obtained at optimized levels of 10.56% glucose, 9.23% sucrose, 0.75% tryptone, 0.446% CaCl₂, and 0.385% K₂HP

SUBMITTER: Afreen A 

PROVIDER: S-EPMC10311403 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature