RawHash: enabling fast and accurate real-time analysis of raw nanopore signals for large genomes.

ABSTRACT: Nanopore sequencers generate electrical raw signals in real-time while sequencing long genomic strands. These raw signals can be analyzed as they are generated, providing an opportunity for real-time genome analysis. An important feature of nanopore sequencing, Read Until, can eject strands from sequencers without fully sequencing them, which provides opportunities to computationally reduce the sequencing time and cost. However, existing works utilizing Read Until either (i) require powerful computational resources that may not be available for portable sequencers or (ii) lack scalability for large genomes, rendering them inaccurate or ineffective. We propose RawHash, the first mechanism that can accurately and efficiently perform real-time analysis of nanopore raw signals for large genome

SUBMITTER: Firtina C 

PROVIDER: S-EPMC10311405 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature