Mapping Antibody Domain Exposure on Nanoparticle Surfaces Using DNA-PAINT.

ABSTRACT: Decorating nanoparticles with antibodies (Ab) is a key strategy for targeted drug delivery and imaging. For this purpose, the orientation of the antibody on the nanoparticle is crucial to maximize fragment antibody-binding (Fab) exposure and thus antigen binding. Moreover, the exposure of the fragment crystallizable (Fc) domain may lead to the engagement of immune cells through one of the Fc receptors. Therefore, the choice of the chemistry for nanoparticle-antibody conjugation is key for the biological performance, and methods have been developed for orientation-selective functionalization. Despite the importance of this issue, there is a lack of direct methods to quantify the antibodies' orientation on the nanoparticle's surface. Here, we present a generic methodology that enables for mu

SUBMITTER: Tholen MME 

PROVIDER: S-EPMC10311592 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature