Thiol-Mediated Uptake of a Cysteine-Containing Nanobody for Anticancer Drug Delivery.

ABSTRACT: The identification of tumor-specific biomarkers is one of the bottlenecks in the development of cancer therapies. Previous work revealed altered surface levels of reduced/oxidized cysteines in many cancers due to overexpression of redox-controlling proteins such as protein disulfide isomerases on the cell surface. Alterations in surface thiols can promote cell adhesion and metastasis, making thiols attractive targets for treatment. Few tools are available to study surface thiols on cancer cells and exploit them for theranostics. Here, we describe a nanobody (CB2) that specifically recognizes B cell lymphoma and breast cancer in a thiol-dependent manner. CB2 binding strictly requires the presence of a nonconserved cysteine in the antigen-binding region and correlates with elevated surface l

SUBMITTER: Goerdeler F 

PROVIDER: S-EPMC10311659 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature