The revealing of a novel double bond reductase related to perilla ketone biosynthesis in Perilla frutescens.

ABSTRACT:

Background

Perilla frutescens is widely used as both a medicine and a food worldwide. Its volatile oils are its active ingredients, and, based on the different volatile constituents, P. frutescens can be divided into several chemotypes, with perilla ketone (PK) being the most common. However, the key genes involved in PK biosynthesis have not yet been identified.

Results

In this study, metabolite constituents and transcriptomic data were compared in leaves of different levels. The variation in PK levels was the opposite of that of isoegoma ketone and egoma ketone in leaves at different levels. Based on transcriptome data, eight candidate genes were identified and successfully expressed in a prokaryotic system. Sequence analysis revealed them to be double bond reductases (PfD

SUBMITTER: Zhou P 

PROVIDER: S-EPMC10311769 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature