Targeting RNA-binding protein HuR to inhibit the progression of renal tubular fibrosis.

ABSTRACT:

Background

Upregulation of an RNA-binding protein HuR has been implicated in glomerular diseases. Herein, we evaluated whether it is involved in renal tubular fibrosis.

Methods

HuR was firstly examined in human kidney biopsy tissue with tubular disease. Second, its expression and the effect of HuR inhibition with KH3 on tubular injury were further assessed in a mouse model induced by a unilateral renal ischemia/reperfusion (IR). KH3 (50 mg kg^-1) was given daily via intraperitoneal injection from day 3 to 14 after IR. Last, one of HuR-targeted pathways was examined in cultured proximal tubular cells.

Results

HuR significantly increases at the site of tubular injury both in progressive CKD in patients and in IR-injured kidneys in mice, accompanied by upregula

SUBMITTER: Huang Z 

PROVIDER: S-EPMC10311833 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature