MiR26-5p inhibits pathological pulmonary microvascular angiogenesis via down-regulating WNT5A.

ABSTRACT:

Objectives

Pathological micro angiogenesis is a key pathogenic factor in pulmonary diseases such as pulmonary hypertension and hepatopulmonary syndrome. More and more pieces of evidence show that excessive proliferation of pulmonary microvascular endothelial cells is the key event of pathological micro angiogenesis. The purpose of this research is to reveal the mechanism of miR26-5p regulating pulmonary microvascular hyperproliferation.

Materials and methods

Hepatopulmonary syndrome rat model was made by common bile duct ligation. HE and IHC staining were used for analysis of the pathology of the rat. CCK8, transwell, and wound healing assay were used to assess miR26-5p or target gene WNT5A functioned toward PMVECs. microRNA specific mimics and inhibitors were used for up/do

SUBMITTER: Chen J 

PROVIDER: S-EPMC10311967 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature