Sophisticated and precise: design and implementation of a real-time optical detection system for ultra-fast PCR.

ABSTRACT: Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) has become indispensable in the realm of disease nucleic acid screening and diagnostics, owing to its remarkable precision and sensitivity, in which the real-time fluorescence detection system plays an extremely critical role. To solve the problems of long time and slow speed of traditional nucleic acid detection, PCR systems are evolving towards ultra-rapid configurations. Nonetheless, most extant ultra-rapid PCR systems either depend on endpoint detection for qualitative assessments due to inherent structural or heating constraints or circumvent the challenge of adapting optical systems to expeditious amplification systems, resulting in potential shortcomings in assay efficacy, volume, or expense. Consequently, this study propose

SUBMITTER: Xi B 

PROVIDER: S-EPMC10312126 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature