Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications.

ABSTRACT: Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of the problem remains largely anecdotal, and as such, feasibility of the goal of at least one potent and specific antibody targeting each protein in a proteome cannot be assessed. Focusing on antibodies for human proteins, we have scaled a standardized characterization approach using parental and knockout cell lines (Laflamme et al., 2019) to assess the performance of 614 commercial antibodies for 65 neuroscience-related proteins. Side-by-side comparisons of all antibodies against each target, obtained from multiple commercial partners, demonstrates that: i) more than 50% of all antibodi

SUBMITTER: Ayoubi R 

PROVIDER: S-EPMC10312534 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature