Foveal RGCs develop altered calcium dynamics weeks after photoreceptor ablation.

ABSTRACT:

Objective or purpose

Physiological changes in retinal ganglion cells (RGCs) have been reported in rodent models of photoreceptor (PR) loss but this has not been investigated in primates. By expressing both a calcium indicator (GCaMP6s) and an optogenetic actuator (ChrimsonR) in foveal RGCs of the macaque, we reactivated RGCs in vivo and assessed their response in the weeks and years following PR loss.

Design

We used an in vivo calcium imaging approach to record optogenetically evoked activity in deafferented RGCs in primate fovea. Cellular scale recordings were made longitudinally over a 10 week period following photoreceptor ablation and compared to responses from RGCs that had lost photoreceptor input more than two years prior.

Participants

Three eyes

SUBMITTER: Xu Z 

PROVIDER: S-EPMC10312553 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature