RCRUX: A Rapid and Versatile Tool for Generating Metabarcoding Reference libraries in R.

ABSTRACT: Key to making accurate taxonomic assignments are curated, comprehensive reference barcode databases. However, the generation and curation of such databases has remained challenging given the large and continuously growing volumes of DNA sequence data and novel reference barcode targets. Monitoring and research applications require a greater diversity of specialized gene regions and targeted taxa to meet taxonomic classification goals then are currently curated by professional staff. Thus, there is a growing need for an easy to implement tool that can generate comprehensive metabarcoding reference libraries for any bespoke locus. We address this need by reimagining CRUX from the Anacapa Toolkit and present the rCRUX package in R. The typical workflow involves searching for plausible seed am

SUBMITTER: Curd EE 

PROVIDER: S-EPMC10312559 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature