A single DPE core promoter motif contributes to in vivo transcriptional regulation and affects cardiac function.

ABSTRACT: Transcription is initiated at the core promoter, which confers specific functions depending on the unique combination of core promoter elements. The downstream core promoter element (DPE) is found in many genes related to heart and mesodermal development. However, the function of these core promoter elements has thus far been studied primarily in isolated, in vitro or reporter gene settings. tinman (tin) encodes a key transcription factor that regulates the formation of the dorsal musculature and heart. Pioneering a novel approach utilizing both CRISPR and nascent transcriptomics, we show that a substitution mutation of the functional tin DPE motif within the natural context of the core promoter results in a massive perturbation of Tinman's regulatory network or

SUBMITTER: Sloutskin A 

PROVIDER: S-EPMC10312617 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature