Dataset Information

Functional glycoproteomics by integrated network assembly and partitioning.

ABSTRACT: The post-translational modification (PTM) of proteins by O-linked β-N-acetyl-D-glucosamine (O-GlcNAcylation) is widespread across the proteome during the lifespan of all multicellular organisms. However, nearly all functional studies have focused on individual protein modifications, overlooking the multitude of simultaneous O-GlcNAcylation events that work together to coordinate cellular activities. Here, we describe Networking of Interactors and SubstratEs (NISE), a novel, systems-level approach to rapidly and comprehensively monitor O-GlcNAcylation across the proteome. Our method integrates affinity purification-mass spectrometry (AP-MS) and site-specific chemoproteomic technologies with network generation and unsupervised partitioning to connect potential upstream regulators with downstream targets of O-GlcNAcylation. The resulting network provides a data-rich framework that reveals both conserved activities of O-GlcNAcylation such as epigenetic regulation as well as tissue-specific functions like synaptic morphology. Beyond O-GlcNAc, this holistic and unbiased systems-level approach provides a broadly applicable framework to study PTMs and discover their diverse roles in specific cell types and biological states.

SUBMITTER: Griffin ME

PROVIDER: S-EPMC10312638 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Functional glycoproteomics by integrated network assembly and partitioning.

Griffin Matthew E ME Thompson John W JW Xiao Yao Y Sweredoski Michael J MJ Aksenfeld Rita B RB Jensen Elizabeth H EH Koldobskaya Yelena Y Schacht Andrew L AL Kim Terry D TD Choudhry Priya P Lomenick Brett B Garbis Spiros D SD Moradian Annie A Hsieh-Wilson Linda C LC

bioRxiv : the preprint server for biology 20230614

The post-translational modification (PTM) of proteins by O-linked β-N-acetyl-D-glucosamine (O-GlcNAcylation) is widespread across the proteome during the lifespan of all multicellular organisms. However, nearly all functional studies have focused on individual protein modifications, overlooking the multitude of simultaneous O-GlcNAcylation events that work together to coordinate cellular activities. Here, we describe Networking of Interactors and SubstratEs (NI ...[more]

PMID: 37398272

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Dataset Information

Functional glycoproteomics by integrated network assembly and partitioning.

Publications

Functional glycoproteomics by integrated network assembly and partitioning.

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