Development of an in situ cell-type specific proteome analysis method using antibody-mediated biotinylation.

ABSTRACT: Since proteins are essential molecules exerting cellular functions, decoding proteome changes is the key to understanding the normal physiology and pathogenesis mechanism of various diseases. However, conventional proteomic studies are often conducted on tissue lumps, in which multiple cell types are entangled, presenting challenges in interpreting the biological dynamics among diverse cell types. While recent cell-specific proteome analysis techniques, like BONCAT, TurboID, and APEX, have emerged, their necessity for genetic modifications limits their usage. The alternative, laser capture microdissection (LCM), although it does not require genetic alterations, is labor-intensive, time-consuming, and requires specialized expertise, making it less suitable for large-scale studies. In this s

SUBMITTER: Ryu T 

PROVIDER: S-EPMC10312661 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature