Dissection and Integration of Bursty Transcriptional Dynamics for Complex Systems.

ABSTRACT: RNA velocity estimation is a potentially powerful tool to reveal the directionality of transcriptional changes in single-cell RNA-seq data, but it lacks accuracy, absent advanced metabolic labeling techniques. We developed a novel approach, TopicVelo, that disentangles simultaneous, yet distinct, dynamics by using a probabilistic topic model, a highly interpretable form of latent space factorization, to infer cells and genes associated with individual processes, thereby capturing cellular pluripotency or multifaceted functionality. Focusing on process-associated cells and genes enables accurate estimation of process-specific velocities via a master equation for a transcriptional burst model accounting for intrinsic stochasticity. The method obtains a global transition matrix by leve

SUBMITTER: Gao CF 

PROVIDER: S-EPMC10312759 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature