The molecular architecture of the desmosomal outer dense plaque by integrative structural modeling.

ABSTRACT: Desmosomes mediate cell-cell adhesion and are prevalent in tissues under mechanical stress. However, their detailed structural characterization is not available. Here, we characterized the molecular architecture of the desmosomal outer dense plaque (ODP) using Bayesian integrative structural modeling via the Integrative Modeling Platform. Starting principally from the structural interpretation of an electron cryo-tomogram, we integrated information from X-ray crystallography, an immuno-electron microscopy study, biochemical assays, in-silico predictions of transmembrane and disordered regions, homology modeling, and stereochemistry information. The integrative structure was validated by information from imaging, tomography, and biochemical studies that were not used in modeli

SUBMITTER: Pasani S 

PROVIDER: S-EPMC10312763 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature