Workflow for shake flask and plate cultivations with fats for polyhydroxyalkanoate bioproduction.

ABSTRACT: Since natural resources for the bioproduction of commodity chemicals are scarce, waste animal fats (WAF) are an interesting alternative biogenic residual feedstock. They appear as by-product from meat production, but several challenges are related to their application: first, the high melting points (up to 60 °C); and second, the insolubility in the polar water phase of cultivations. This leads to film and clump formation in shake flasks and microwell plates, which inhibits microbial consumption. In this study, different flask and well designs were investigated to identify the most suitable experimental set-up and further to create an appropriate workflow to achieve the required reproducibility of growth and product synthesis. The dissolved oxygen concentration was measured in-line through

SUBMITTER: Riedel SL 

PROVIDER: S-EPMC10313549 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature