Small RNA sequencing of field Culex mosquitoes identifies patterns of viral infection and the mosquito immune response.

ABSTRACT: Mosquito-borne disease remains a significant burden on global health. In the United States, the major threat posed by mosquitoes is transmission of arboviruses, including West Nile virus by mosquitoes of the Culex genus. Virus metagenomic analysis of mosquito small RNA using deep sequencing and advanced bioinformatic tools enables the rapid detection of viruses and other infecting organisms, both pathogenic and non-pathogenic to humans, without any precedent knowledge. In this study, we sequenced small RNA samples from over 60 pools of Culex mosquitoes from two major areas of Southern California from 2017 to 2019 to elucidate the virome and immune responses of Culex. Our results demonstrated that small RNAs not only allowed the detection of viruses but also revealed distinct patterns of vi

SUBMITTER: Abel SM 

PROVIDER: S-EPMC10313667 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature