Developing and deploying an efficient genotyping workflow for accelerating maize improvement in developing countries.

ABSTRACT: Background: Molecular breeding is an essential tool for accelerating genetic gain in crop improvement towards meeting the need to feed an ever-growing world population. Establishing low-cost, flexible genotyping platforms in small, public and regional laboratories can stimulate the application of molecular breeding in developing countries. These laboratories can serve plant breeding projects requiring low- to medium-density markers for marker-assisted selection (MAS) and quality control (QC) activities. Methods: We performed two QC and MAS experiments consisting of 637 maize lines, using an optimised genotyping workflow involving an in-house competitive allele-specific PCR (KASP) genotyping system with an optimised sample collection, preparation, and DNA extraction and quanti

SUBMITTER: Offornedo Q 

PROVIDER: S-EPMC10313903 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature