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Integrated spatial transcriptome and metabolism study reveals metabolic heterogeneity in human injured brain.

ABSTRACT: Single-cell transcriptomics can provide quantitative molecular signatures for large, unbiased samples of the diverse cell types in the brain. With the advances of multi-omics datasets, a major challenge is to validate and integrate results into a biological understanding of spatial organization and functional orientation. Here, we generate spatial transcriptomes and metabolites from six patients with brain trauma with surgical samples. The resulting spatial marker gene, which is highly replicable across analysis methods, sequencing technologies, and modalities, is a comprehensive molecular marker of the diverse metabolic changes in human injured brains. The atlas includes an area of lipid peroxidation that resembles injured neurons in the brain. We further discover imbalanced myo-inositol and myo-inositol phosphate and related spatial markers. Our results highlight the complex transcriptomic regulation and metabolic alterations in the injured brain and will directly enable the design of reagents to target specific genes in the human brain for functional analysis.

SUBMITTER: Zheng P

PROVIDER: S-EPMC10313933 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Integrated spatial transcriptome and metabolism study reveals metabolic heterogeneity in human injured brain.

Zheng Ping P Zhang Ning N Ren Dabin D Yu Cong C Zhao Bin B Zhang Yisong Y

Cell reports. Medicine 20230531 6

Single-cell transcriptomics can provide quantitative molecular signatures for large, unbiased samples of the diverse cell types in the brain. With the advances of multi-omics datasets, a major challenge is to validate and integrate results into a biological understanding of spatial organization and functional orientation. Here, we generate spatial transcriptomes and metabolites from six patients with brain trauma with surgical samples. The resulting spatial marker gene, which is highly replicabl ...[more]

PMID: 37263268

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Dataset Information

Integrated spatial transcriptome and metabolism study reveals metabolic heterogeneity in human injured brain.

Publications

Integrated spatial transcriptome and metabolism study reveals metabolic heterogeneity in human injured brain.

Similar Datasets

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