Activated regulatory T-cells promote duodenal bacterial translocation into necrotic areas in severe acute pancreatitis.

ABSTRACT:

Objective

In acute pancreatitis (AP), bacterial translocation and subsequent infection of pancreatic necrosis are the main risk factors for severe disease and late death. Understanding how immunological host defence mechanisms fail to protect the intestinal barrier is of great importance in reducing the mortality risk of the disease. Here, we studied the role of the T_reg/Th17 balance for maintaining the intestinal barrier function in a mouse model of severe AP.

Design

AP was induced by partial duct ligation in C57Bl/6 or DEREG mice, in which regulatory T-cells (T_reg) were depleted by intraperitoneal injection of diphtheria toxin. By flow cytometry, functional suppression assays and transcriptional profiling we analysed T_reg activation and cha

SUBMITTER: Glaubitz J 

PROVIDER: S-EPMC10314084 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature