The role of Dmnt1 during spermatogenesis of the insect Oncopeltus fasciatus.

ABSTRACT:

Background

The function of DNA methyltransferase genes of insects is a puzzle, because an association between gene expression and methylation is not universal for insects. If the genes normally involved in cytosine methylation are not influencing gene expression, what might be their role? We previously demonstrated that gametogenesis of Oncopeltus fasciatus is interrupted at meiosis following knockdown of DNA methyltransferase 1 (Dnmt1) and this is unrelated to changes in levels of cytosine methylation. Here, using transcriptomics, we tested the hypothesis that Dmnt1 is a part of the meiotic gene pathway. Testes, which almost exclusively contain gametes at varying stages of development, were sampled at 7 days and 14 days following knockdown of Dmnt1 using RNAi.

Results

Using

SUBMITTER: Cunningham CB 

PROVIDER: S-EPMC10314392 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature