Establishing a Robust and Reliable Response from a Potent Osmium-Based Photosensitizer Via Lipid Nanoformulation^†.

ABSTRACT: Osmium (Os) based photosensitizers (PSs) are a unique class of nontetrapyrrolic metal-containing PSs that absorb red light. We recently reported a highly potent Os(II) PS, rac-[Os(phen)₂ (IP-4T)](Cl)₂ , referred to as ML18J03 herein, with light EC₅₀ values as low as 20 pm. ML18J03 also exhibits low dark toxicity and submicromolar light EC₅₀ values in hypoxia in some cell lines. However, owing to its longer oligothiophene chain, ML18J03 is not completely water soluble and forms 1-2 μm sized aggregates in PBS containing 1% DMSO. This aggregation causes variability in PDT efficacy between assays and thus unreliable and irreproducible reports of in vitro activity. To that end, we utilized PEG-modified DPPC liposomes (138 nm diameter) and DSPE-mPEG

SUBMITTER: Cole HD 

PROVIDER: S-EPMC10315168 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature