Discovery of 14-3-3 zeta as a potential biomarker for cardiac hypertrophy.

ABSTRACT: Acute myocardial infarction (AMI) is a multifaceted syndrome influenced by the functions of various extrinsic and intrinsic pathways and pathological processes, which can be detected in circulation using biomarkers. In this study, we investigated the secretome protein profile of induced-hypertrophy cardiomyocytes to identify next-generation biomarkers for AMI diagnosis and management. Hypertrophy was successfully induced in immortalized human cardiomyocytes (T0445) by 200 nM ET-1 and 1 μM Ang II. The protein profiles of hypertrophied cardiomyocyte secretomes were analyzed by nano-liquid chromatography with tandem mass spectrometry and differentially expressed proteins that have been identified by Ingenuity Pathway Analysis. The levels of 32 proteins increased significantly (＞1.4 fold), whe

SUBMITTER: Mahmud J 

PROVIDER: S-EPMC10315568 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature