Technical upgrade of an open-source liquid handler to support bacterial colony screening.

ABSTRACT: The optimization of genetically engineered biological constructs is a key step to deliver high-impact biotechnological applications. The use of high-throughput DNA assembly methods allows the construction of enough genotypic variants to successfully cover the target design space. This, however, entails extra workload for researchers during the screening stage of candidate variants. Despite the existence of commercial colony pickers, their high price excludes small research laboratories and budget-adjusted institutions from accessing such extensive screening capability. In this work we present COPICK, a technical solution to automatize colony picking in an open-source liquid handler Opentrons OT-2. COPICK relies on a mounted camera to capture images of regular Petri dishes and detect microb

SUBMITTER: Del Olmo Lianes I 

PROVIDER: S-EPMC10315574 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature