Barley stripe mosaic virus-mediated somatic and heritable gene editing in barley (Hordeum vulgare L.).

ABSTRACT: Genome editing strategies in barley (Hordeum vulgare L.) typically rely on Agrobacterium-mediated genetic transformation for the delivery of required genetic reagents involving tissue culture techniques. These approaches are genotype-dependent, time-consuming, and labor-intensive, which hampers rapid genome editing in barley. More recently, plant RNA viruses have been engineered to transiently express short guide RNAs facilitating CRISPR/Cas9-based targeted genome editing in plants that constitutively express Cas9. Here, we explored virus-induced genome editing (VIGE) based on barley stripe mosaic virus (BSMV) in Cas9-transgenic barley. Somatic and heritable editing in the ALBOSTRIANS gene (CMF7) resulting in albino/variegated chloroplast-defective

SUBMITTER: Tamilselvan-Nattar-Amutha S 

PROVIDER: S-EPMC10315673 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature