Obesity and PCOS radically alters the snRNA composition of follicular fluid extracellular vesicles.

ABSTRACT:

Introduction

The ovarian follicle consists of the oocyte, somatic cells, and follicular fluid (FF). Proper signalling between these compartments is required for optimal folliculogenesis. The association between polycystic ovarian syndrome (PCOS) and extracellular vesicular small non-coding RNAs (snRNAs) signatures in follicular fluid (FF) and how this relates to adiposity is unknown. The purpose of this study was to determine whether FF extracellular vesicle (FFEV)-derived snRNAs are differentially expressed (DE) between PCOS and non-PCOS subjects; and if these differences are vesicle-specific and/or adiposity-dependent.

Methods

FF and granulosa cells (GC) were collected from 35 patients matched by demographic and stimulation parameters. FFEVs were isolated and snRNA librari

SUBMITTER: Wyse BA 

PROVIDER: S-EPMC10315679 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature