Induction of endoplasmic reticulum stress is associated with the anti-tumor activity of monepantel across cancer types.

ABSTRACT:

Background

Monepantel is an anti-helminthic drug that also has anti-cancer properties. Despite several studies over the years, the molecular target of monepantel in mammalian cells is still unknown, and its mechanism-of-action is not fully understood, though effects on cell cycle, mTOR signalling and autophagy have been implicated.

Methods

Viability assays were performed on >20 solid cancer cell cells, and apoptosis assays were performed on a subset of these, including 3D cultures. Genetic deletion of BAX/BAK and ATG were used to establish roles of apoptosis and autophagy in killing activity. RNA-sequencing was performed on four cell lines after monepantel treatment, and differentially regulated genes were confirmed by Western blotting.

Results

We showed that monepant

SUBMITTER: Harris TJ 

PROVIDER: S-EPMC10315739 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature