Action of econazole on Ca²⁺ levels and cytotoxicity in OC2 human oral cancer cells.

ABSTRACT:

Background/purpose

Econazole is an antifungal drug. Antifungal activity of econazole against non-dermatophyte molds was reported. Econazole inhibited Ca²⁺ channels and stimulated cytotoxicity in lymphoma and leukemia cells. Ca²⁺ cations are crucial second envoy that triggers various processes. This research was aimed to investigate action of econazole on Ca²⁺ levels and cytotoxicity in OC2 human oral cancer cells.

Materials and methods

Cytosolic Ca²⁺ levels ([Ca²⁺]_i) were detected employing fura-2 as a probe in a RF-5301PC spectrofluorophotometer (Shimadzu). Cytotoxicity was determined using 4-[3-[4-lodophenyl]-2-4(4-nitrophenyl)-2H-5-tetrazolio-1,3-benzene disulfonate] (WST-1) to detect fluorescence changes.<

SUBMITTER: Wang JL 

PROVIDER: S-EPMC10316490 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature