Secretory carrier-associated membrane protein 5 regulates cell-surface targeting of T-type calcium channels.

ABSTRACT: Missense mutations in the human secretary carrier-associated membrane protein 5 (SCAMP5) cause a variety of neurological disorders including neurodevelopmental delay, epilepsy, and Parkinson's disease. We recently documented the importance of SCAMP2 in the regulation of T-type calcium channel expression in the plasma membrane. Here, we show that similar to SCAMP2, the co-expression of SCAMP5 in tsA-201 cells expressing recombinant Ca_v3.1, Ca_v3.2, and Ca_v3.3 channels nearly abolished whole-cell T-type currents. Recording of intramembrane charge movements revealed that SCAMP5-induced inhibition of T-type currents is primarily caused by the reduced expression of functional channels in the plasma membrane. Moreover, we show that SCAMP5-mediated downregulation o

SUBMITTER: Mustafa ER 

PROVIDER: S-EPMC10316736 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature