The effect of semen cuscutae flavonoid on Sertoli cells and blood-testis barrier in male infertility: integrating network pharmacology and experimental verification.

ABSTRACT:

Context

Semen cuscutae is commonly used to treat male infertility (MI), and semen cuscutae flavonoid (SCF) is the main active component of semen cuscutae. The therapeutic mechanism of SCF on MI is still unclear.

Objective

To clarify the mechanisms of SCF against MI.

Materials and methods

Network pharmacology and molecular docking were used to predict the potential pathways of SCF against MI. Primary Sertoli cells (SCs) were extracted from testis of 60-day-old rats and divided into Control, Model, and 3 treatment groups. The Control and Model groups were given normal medium, the treatment groups were treated with various concentrations of SCF-containing medium (200, 400, and 800 μg/mL). After 24 h, the Model and treatment groups were exposed to heat stress at 43 °C for

SUBMITTER: Liu CX 

PROVIDER: S-EPMC10316745 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature