CRISPR-Cas9-based repeat depletion for high-throughput genotyping of complex plant genomes.

ABSTRACT: High-throughput genotyping enables the large-scale analysis of genetic diversity in population genomics and genome-wide association studies that combine the genotypic and phenotypic characterization of large collections of accessions. Sequencing-based approaches for genotyping are progressively replacing traditional genotyping methods because of the lower ascertainment bias. However, genome-wide genotyping based on sequencing becomes expensive in species with large genomes and a high proportion of repetitive DNA. Here we describe the use of CRISPR-Cas9 technology to deplete repetitive elements in the 3.76-Gb genome of lentil (Lens culinaris), 84% consisting of repeats, thus concentrating the sequencing data on coding and regulatory regions (single-copy regions). We designed a custom

SUBMITTER: Rossato M 

PROVIDER: S-EPMC10317117 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature