Characterizing the targets of transcription regulators by aggregating ChIP-seq and perturbation expression data sets.

ABSTRACT: Mapping the gene targets of chromatin-associated transcription regulators (TRs) is a major goal of genomics research. ChIP-seq of TRs and experiments that perturb a TR and measure the differential abundance of gene transcripts are a primary means by which direct relationships are tested on a genomic scale. It has been reported that there is a poor overlap in the evidence across gene regulation strategies, emphasizing the need for integrating results from multiple experiments. Although research consortia interested in gene regulation have produced a valuable trove of high-quality data, there is an even greater volume of TR-specific data throughout the literature. In this study, we show a workflow for the identification, uniform processing, and aggregation of ChIP-seq and TR perturbation exp

SUBMITTER: Morin A 

PROVIDER: S-EPMC10317128 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature