Molecular mechanisms of processive glycoside hydrolases underline catalytic pragmatism.

ABSTRACT: Processive and distributive catalysis defines the conversion continuum, thus underpinning the transformation of oligo- and polymeric substrates by enzymes. Distributive catalysis follows an association-transformation-dissociation pattern during the formation of enzyme-reactant complexes, whereas during processive catalysis, enzymes partner with substrates and complete multiple catalytic events before dissociation from an enzyme-substrate complex. Here, we focus on processive catalysis in glycoside hydrolases (GHs), which ensures efficient conversions of substrates with high precision, and has the advantage over distributive catalysis in efficiency. The work presented here examines a recent discovery of substrate-product-assisted processive catalysis in the GH3 family enzymes with enclosed

SUBMITTER: Hrmova M 

PROVIDER: S-EPMC10317168 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature