Automated identification and tracking of cells in Cytometry of Reaction Rate Constant (CRRC).

ABSTRACT: Cytometry of Reaction Rate Constant (CRRC) is a method for studying cell-population heterogeneity using time-lapse fluorescence microscopy, which allows one to follow reaction kinetics in individual cells. The current and only CRRC workflow utilizes a single fluorescence image to manually identify cell contours which are then used to determine fluorescence intensity of individual cells in the entire time-stack of images. This workflow is only reliable if cells maintain their positions during the time-lapse measurements. If the cells move, the original cell contours become unsuitable for evaluating intracellular fluorescence and the CRRC experiment will be inaccurate. The requirement of invariant cell positions during a prolonged imaging is impossible to satisfy for motile cells. Here we re

SUBMITTER: Nebbioso G 

PROVIDER: S-EPMC10317225 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature