Identification of mixed and successive blood meals of mosquitoes using MALDI-TOF MS protein profiling.

ABSTRACT:

Background

The accurate and rapid identification of mosquito blood meals is critical to study the interactions between vectors and vertebrate hosts and, subsequently, to develop vector control strategies. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling has been shown to be a reliable and effective tool for identifying single blood meals from mosquitoes.

Methods

In this study, we developed MALDI-TOF MS profiling protocols to identify Anopheles gambiae Giles, Anopheles coluzzii and Aedes albopictus mosquitoes' mixed blood meals and the last of successive blood meals. The mosquitoes were either successively artificially fed with distinct host bloods or engorged with mixed bloods from distinct vertebrate hosts, such

SUBMITTER: Tandina F 

PROVIDER: S-EPMC10317667 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature