Disruption of cortical cell type composition and function underlies diabetes-associated cognitive decline.

ABSTRACT:

Aims/hypothesis

Type 2 diabetes is associated with increased risk of cognitive decline although the pathogenic basis for this remains obscure. Deciphering diabetes-linked molecular mechanisms in cells of the cerebral cortex could uncover novel therapeutic targets.

Methods

Single-cell transcriptomic sequencing (scRNA-seq) was conducted on the cerebral cortex in a mouse model of type 2 diabetes (db/db mice) and in non-diabetic control mice in order to identify gene expression changes in distinct cell subpopulations and alterations in cell type composition. Immunohistochemistry and metabolic assessment were used to validate the findings from scRNA-seq and to investigate whether these cell-specific dysfunctions impact the neurovascular unit (NVU). Furthermore, the behavioural an

SUBMITTER: Little K 

PROVIDER: S-EPMC10317904 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature