Development and characterization of an intra-articular fracture mediated model of post-traumatic osteoarthritis.

ABSTRACT:

Purpose

This study aimed to develop and characterize a closed intra-articular fracture (IAF) mediated post-traumatic osteoarthritis (PTOA) model in rats to serve as a testbed for putative disease modifying interventions.

Methods

Male rats were subject to a 0 Joule (J), 1 J, 3 J, or 5 J blunt-force impact to the lateral aspect of the knee and allowed to heal for 14 and 56 days. Micro-CT was performed at time of injury and at the specified endpoints to assess bone morphometry and bone mineral density measurements. Cytokines and osteochondral degradation markers were assayed from serum and synovial fluid via immunoassays. Histopathological analyses were performed on decalcified tissues and assessed for evidence of osteochondral degradation.

Results

High-energy (5 J) blun

SUBMITTER: Valerio MS 

PROVIDER: S-EPMC10317931 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature