Human and porcine aortic valve endothelial and interstitial cell isolation and characterization

ABSTRACT:

Background

Calcific aortic valve stenosis (AVS) is defined by pathological changes in the aortic valve (AV) and their predominant cell types: valvular interstitial (VICs) and endothelial cells (VECs). Understanding the cellular and molecular mechanisms of this disease is a prerequisite to identify potential pharmacological treatment strategies. In this study, we present a unique aortic valve cell isolation technique to acquire specific human and porcine cell populations and compared VICs and VECs of these species with each other for the first time.

Methods

AV cells were isolated from tissue obtained from human patients undergoing surgical aortic valve replacement (SAVR) or from porcine hearts. Functional analysis and in vitro experiments revealed that endothelial-to-mesen

SUBMITTER: Nehl D 

PROVIDER: S-EPMC10318150 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature