Enabling oxygen-controlled microfluidic cultures for spatiotemporal microbial single-cell analysis.

ABSTRACT: Microfluidic cultivation devices that facilitate O₂ control enable unique studies of the complex interplay between environmental O₂ availability and microbial physiology at the single-cell level. Therefore, microbial single-cell analysis based on time-lapse microscopy is typically used to resolve microbial behavior at the single-cell level with spatiotemporal resolution. Time-lapse imaging then provides large image-data stacks that can be efficiently analyzed by deep learning analysis techniques, providing new insights into microbiology. This knowledge gain justifies the additional and often laborious microfluidic experiments. Obviously, the integration of on-chip O₂ measurement and control during the already complex microfluidic cultivation, and the develo

SUBMITTER: Kasahara K 

PROVIDER: S-EPMC10318409 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature