Vibrio-Sequins - dPCR-traceable DNA standards for quantitative genomics of Vibrio spp.

ABSTRACT:

Background

Vibrio spp. are a diverse group of ecologically important marine bacteria responsible for several foodborne outbreaks of gastroenteritis around the world. Their detection and characterization are moving away from conventional culture-based methods towards next generation sequencing (NGS)-based approaches. However, genomic methods are relative in nature and suffer from technical biases arising from library preparation and sequencing. Here, we introduce a quantitative NGS-based method that enables the quantitation of Vibrio spp. at the limit of quantification (LOQ) through artificial DNA standards and their absolute quantification via digital PCR (dPCR).

Results

We developed six DNA standards, called Vibrio-Sequins, together with optimized TaqMan assays for their qu

SUBMITTER: Flutsch S 

PROVIDER: S-EPMC10318669 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature