Optimized screening of DNA methylation sites combined with gene expression analysis to identify diagnostic markers of colorectal cancer.

ABSTRACT:

Background

The prognosis of patients with colorectal cancer is related to early detection. However, commonly used screening markers lack sensitivity and specificity. In this study, we identified diagnostic methylation sites for colorectal cancer.

Methods

After screening the colorectal cancer methylation dataset, diagnostic sites were identified via survival analysis, difference analysis, and ridge regression dimensionality reduction. The correlation between the selected methylation sites and the estimation of immune cell infiltration was analyzed. The accuracy of the diagnosis was verified using different datasets and the 10-fold crossover method.

Results

According to Gene Ontology, the main enrichment pathways of genes with hypermethylation sites are axon development

SUBMITTER: Ye Z 

PROVIDER: S-EPMC10318760 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature